Avaliação in vitro da viabilidade de células tumorais após tratamento com α–terpineol
Carregando...
Data
2018-01
Autores
Orientador
Goettert, Marcia Ines
Banca
Título do periódico
ISSN
Título do Volume
Editor
Resumo
Os tratamentos atuais para o câncer causam prejuízos para o indivíduo, pois além das células tumorais também atingem as demais células em crescimento, ocasionando em diversos efeitos adversos. Os produtos naturais apresentam grande potencial para o desenvolvimento de novos fármacos e com reduzidos efeitos adversos. O composto α–terpineol apresentou atividade antiproliferativa significativa após tratamento de linhagens tumorais humanas in vitro, sugerindo ser uma molécula bioativa candidata ou coadjuvante para a terapêutica do câncer. Para a avaliação in vitro da viabilidade celular de diferentes linhagens de células tumorais foi utilizado o ensaio colorimétrico MTT. Observou-se que o composto reduziu a viabilidade celular de maneira significativa em duas das cinco linhagens avaliadas, adenocarcinoma colorretal, HT-29 (75,5% ± 2,17) e adenocarcinoma de mama MCF-7 (74% ± 1,15). Nas linhagens de adenocarcinoma colorretal (Caco-2), adenocarcinoma de próstata (LNCaP) e adenocarcinoma gástrico (ACP03), a viabilidade celular não apresentou redução significativa. Estes dados são importantes para o direcionamento de estudos de possíveis biomoléculas com potencial terapêutico. Contudo, ainda são necessários estudos para avaliar o mecanismo de ação do α-terpineol e também seu uso em outras linhagens celulares.
The treatments used nowadays for cancer may bring some losses to the person, because the tumoral cells they reach other cells which are growing, what might cause many different collateral effects. Natural products show big potential to the developing of new drugs with lowered collateral effects. The compound α-terpineol showed significant antiproliferative activity after the treatment of human tumoral lineages in vitro, what suggest it is a bioactive competitor molecule or a supporting molecule to the therapy against cancer. To the in vitro evaluation of viability from different lineages of tumoral cells which was used to the colorimetric test MTT. From this test it could be seen that the compound reduced the cell viability significantly in two of five lineages evaluated, colorectal adenocarcinoma, HT-29 (75,5% ± 2,17) e breast adenocarcinoma MCF-7 (74% ± 1,15). In an adenocarcinoma rectal lineage (Caco-2), prostate adenocarcinoma (LNCaP) and gastric adenocarcinoma (ACP03) the cell’s viability did not show significant reduce. This data is important to the targeting of bio molecular studies with therapeutic potential. However some studies are still needed to evaluate the mechanism of action from α- terpineol and also its use in other cell leneages.
The treatments used nowadays for cancer may bring some losses to the person, because the tumoral cells they reach other cells which are growing, what might cause many different collateral effects. Natural products show big potential to the developing of new drugs with lowered collateral effects. The compound α-terpineol showed significant antiproliferative activity after the treatment of human tumoral lineages in vitro, what suggest it is a bioactive competitor molecule or a supporting molecule to the therapy against cancer. To the in vitro evaluation of viability from different lineages of tumoral cells which was used to the colorimetric test MTT. From this test it could be seen that the compound reduced the cell viability significantly in two of five lineages evaluated, colorectal adenocarcinoma, HT-29 (75,5% ± 2,17) e breast adenocarcinoma MCF-7 (74% ± 1,15). In an adenocarcinoma rectal lineage (Caco-2), prostate adenocarcinoma (LNCaP) and gastric adenocarcinoma (ACP03) the cell’s viability did not show significant reduce. This data is important to the targeting of bio molecular studies with therapeutic potential. However some studies are still needed to evaluate the mechanism of action from α- terpineol and also its use in other cell leneages.
Descrição
Palavras-chave
Compostos naturais; Câncer; α-terpineol; MTT
Citação
DAIPRAI, Gabriela. Avaliação in vitro da viabilidade de células tumorais após tratamento com α–terpineol. 2017. Artigo (Graduação) – Curso de Biomedicina, Universidade do Vale do Taquari - Univates, Lajeado, 22 jun. 2017. Disponível em: http://hdl.handle.net/10737/1842.