Avaliação microbiológica e morfológica do resíduo gerado pelo microagulhamento, antes e depois do protocolo de desinfecção
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Data
2021-06
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Stülp, Simone
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Essa busca incessante pela beleza aumenta a procura por procedimentos na área da estética, e o com a finalidade de rejuvenescimento tissular, o microagulhamento vem ganhando destaque nesta área. Junto com esse fenômeno, é normal um crescimento no que se diz respeito a geração de resíduos, e segundo a Associação Brasileira de Empresas de Limpeza Pública e Resíduos Especiais (ABRELPE), 36% dos resíduos gerados são destinados ao descarte sem nenhum tratamento prévio, demonstrando sérios riscos à saúde pública, aos trabalhadores, ao meio ambiente, e ainda, violando as normas existentes. Neste sentido, o presente trabalho teve os seguintes objetivos: avaliar a contaminação microbiológica do resíduo gerado pela técnica de microagulhamento, investigar a efetividade de um protocolo de desinfecção no que diz respeito à contaminação microbiológica, e também, analisar os possíveis danos estruturais no dispositivo de microagulhamento advindos de sua utilização clínica e de protocolos de desinfecção. Utilizou-se como amostra para tal investigação onze resíduos de equipamentos de microagulhamento que foram coletados imediatamente após seu uso clínico, e antes de seu descarte em lixo infectado perfurocortante. Para a análise microbiológica do resíduo utilizou- se placas de petri com os meios de cultura Ágar-sangue e MacConkey, tais resíduos foram divididos em 2 grupos, o grupo S/I que não sofreu nenhuma intervenção de desinfecção e o I que foi submetido ao procedimento operacional padrão de desinfecção. Foi realizado a análise por Microscopia Eletrônica de Varredura (MEV) para investigar possíveis danos no equipamento após seu uso e sua submissão às intervenções de desinfecção, sendo que, elaborou- se quatro grupos para efeito de comparação : grupo controle (C), composto por equipamento sem nenhuma manipulação; grupo sem intervenção de desinfecção (S/I), que teve apenas contato com a pele do paciente; aparelho submetido ao procedimento operacional de desinfecção (I) após ter tido contato com a pele do paciente; e grupo (A), dispositivo de microagulhamento submetido ao autoclave após ter tido contato com a pele do paciente e passado pelo procedimento operacional de desinfecção. Os resultados mostraram que a técnica de microagulhamento gera rejeito com potencial infectante relevante, uma vez que a cultura microbiológica apontou a presença de Staphylococcus epidermidis no equipamento após sua utilização com finalidade de tratamento estético facial. Além do mais, pode-se evidenciar que o protocolo de desinfecção proposto foi efetivo neste modelo experimental, promovendo dano mínimo ao dispositivo, quando comparado com a autoclave.
This relentless search for beauty increases the demand for procedures in the aesthetic area, and for the purpose of tissue rejuvenation, microneedling has been gaining prominence in this area. Along with this phenomenon, growth in the generation of waste is normal, and according to the Brazilian Association of Public Cleaning and Special Waste Companies (ABRELPE), 36% of the waste generated is destined for disposal without any prior treatment, demonstrating serious risks to public health, workers, the environment, and even violating existing rules. In this sense, the present work had the following objectives: to evaluate the microbiological contamination of the residue generated by the microneedling technique, to investigate the effectiveness of a disinfection protocol with regard to microbiological contamination, and also, to analyze the possible structural damages in the device of microneedling arising from its clinical use and disinfection protocols. Eleven residues of microneedling equipment that were collected immediately after their clinical use, and before being disposed of in sharps infected garbage, were used as a sample for this investigation. For the microbiological analysis of the residue, petri dishes were used with the culture media Agar-blood and MacConkey, such residues were divided into 2 groups for better methodological understanding, the S / I group that did not undergo any disinfection intervention and the I that has undergone the standard operating disinfection procedure.The analysis was performed by Scanning Electron Microscopy (SEM) to investigate possible damage to the equipment after its use and its submission to disinfection interventions, seeing that four groups were elaborated for comparison: control group (C), composed of equipment without any manipulation; group without disinfection intervention (S / I), which had only contact with the patient's skin; device submitted to the operational disinfection procedure (I) after having had contact with the patient's skin; and group (A), microneedling device submitted to the autoclave after having had contact with the patient's skin and undergoing the operational disinfection procedure.The results showed that the microneedling technique generates tailings with a relevant infectious potential, since the microbiological culture indicated the presence of Staphylococcus epidermidis in the equipment after its use for the purpose of aesthetic facial treatment. Furthermore, it can be seen that the proposed disinfection protocol was effective in this experimental model, promoting minimal damage to the device, when compared to the autoclave.
This relentless search for beauty increases the demand for procedures in the aesthetic area, and for the purpose of tissue rejuvenation, microneedling has been gaining prominence in this area. Along with this phenomenon, growth in the generation of waste is normal, and according to the Brazilian Association of Public Cleaning and Special Waste Companies (ABRELPE), 36% of the waste generated is destined for disposal without any prior treatment, demonstrating serious risks to public health, workers, the environment, and even violating existing rules. In this sense, the present work had the following objectives: to evaluate the microbiological contamination of the residue generated by the microneedling technique, to investigate the effectiveness of a disinfection protocol with regard to microbiological contamination, and also, to analyze the possible structural damages in the device of microneedling arising from its clinical use and disinfection protocols. Eleven residues of microneedling equipment that were collected immediately after their clinical use, and before being disposed of in sharps infected garbage, were used as a sample for this investigation. For the microbiological analysis of the residue, petri dishes were used with the culture media Agar-blood and MacConkey, such residues were divided into 2 groups for better methodological understanding, the S / I group that did not undergo any disinfection intervention and the I that has undergone the standard operating disinfection procedure.The analysis was performed by Scanning Electron Microscopy (SEM) to investigate possible damage to the equipment after its use and its submission to disinfection interventions, seeing that four groups were elaborated for comparison: control group (C), composed of equipment without any manipulation; group without disinfection intervention (S / I), which had only contact with the patient's skin; device submitted to the operational disinfection procedure (I) after having had contact with the patient's skin; and group (A), microneedling device submitted to the autoclave after having had contact with the patient's skin and undergoing the operational disinfection procedure.The results showed that the microneedling technique generates tailings with a relevant infectious potential, since the microbiological culture indicated the presence of Staphylococcus epidermidis in the equipment after its use for the purpose of aesthetic facial treatment. Furthermore, it can be seen that the proposed disinfection protocol was effective in this experimental model, promoting minimal damage to the device, when compared to the autoclave.
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Palavras-chave
Biossegurança; Estética; Resíduos; Biosafety; Aesthetics; Residue
Citação
ROGÉRI, Luana Nicolau. Avaliação microbiológica e morfológica do resíduo gerado pelo microagulhamento, antes e depois do protocolo de desinfecção. 2021. Dissertação (Mestrado) – Curso de Sistemas Ambientais Sustentáveis, Universidade do Vale do Taquari - Univates, Lajeado, 05 maio 2021. Disponível em: http://hdl.handle.net/10737/3154.