Avaliação do potencial alergênico da proteína Tyr p2 do ácaro de de importância médica Tyrophagus putrescentiae
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Data
2025-02-17
Autores
Orientador
Timmers, Luis Fernando Saraiva Macedo
Banca
Henriques, João Antonio Pêgas
Lapinscki, Bruna Amaral
Bachega, José Fernando Ruggiero
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Resumo
Nos últimos anos, os processos alérgicos e respiratórios têm sido atrelados às doenças crônicas respiratórias, que em sua maioria são preveníveis e tratáveis. Dentre estas situações patológicas são comumente frequentes as rinites, asma brônquica, doença pulmonar obstrutiva crônica – DPOC, rinomodelamento por produção exacerbada de muco por questões envolvendo mucosa epitelial do aparelho respiratório superior e inferior. A partir destas situações, há uma exacerbada capacidade inflamatória mediada pela cascata de citocinas; onde enfatizamos o Fator de Necrose Tumoral (TNF-α). Este processo da cascata inflamatória apresenta em sua grande maioria intercorrências como inflamação persistente na via aérea, remodelamento do tecido devido à fibrose subepitelial aumentando a permeabilidade de peptídeos agressores, espessamento da musculatura lisa, à queratinização da célula e, por fim, hiperplasia das células do trato respiratório, reduzindo a qualidade de vida desses indivíduos acometidos. Neste contexto patológico de amplitude mundial, os ácaros têm sido amplamente vinculados ao desenvolvimento das doenças alérgicas. Estão presentes na poeira doméstica, ambiente. Fazendo assim, com que os ácaros sejam mundialmente reconhecidos como alergênicos, bem como a proteína das células oriundas dos dejetos do trato intestinal desta fauna. Para tanto, se faz necessário identificar sequências de peptídeos com capacidade alergênica na proteína Tyr p2 de Tyrophagus putrescentiae. A predição dos peptídeos potencialmente alergênicos, bem como a avaliação da energia de interação destes com a IgE, foi realizada por meio de ferramentas computacionais - docking molecular. As interações permitiram a síntese dos peptídeos preditos como de maior capacidade alergênica, para que futuramente sejam submetidos a ensaios celulares permitindo se observar a cascata inflamatória gerada pelos peptídeos preditos.
Allergic and respiratory processes have been linked to chronic respiratory diseases, most of which are preventable and treatable. Among these pathological conditions, rhinitis, bronchial asthma, chronic obstructive pulmonary disease, or COPD, and rhinomodeling due to exacerbated mucus production involving the epithelial mucosa of the upper and lower respiratory tract are commonly encountered. From these conditions, there is an exacerbated inflammatory capacity mediated by the cytokine cascade, with an emphasis on Tumor Necrosis Factor-α (TNF-α). This inflammatory cascade process predominantly presentes interferences such as persistent airway inflammation, tissue remodeling due to subepithelial fibrosis increasing the permeability of aggressive peptides, smooth muscle thickening, cell keratinization, and ultimately, respiratory tract cell hyperplasia, reducing the quality of life of affected individuals. In this worldwide pathological context, mites have been widely implicated in the development of allergic diseases. They are present in household dust environments, thus making mites globally recognized as allergens, as well as the protein from cells originating from the intestinal tract of this fauna's waste. Therefore, it is necessary to identify peptide sequences with allergenic capacity in the Tyr p2 protein of Tyrophagus putrescentiae. The prediction of potentially allergenic peptides, as well as the evaluation of their interaction energy with IgE, was performed through computational tools – molecular docking. These interactions allowed the synthesis of peptides predicted to have higher allergenic capacity, to be subsequently subjected to cellular assays to observe the inflammatory cascade generated by the predicted peptides
Allergic and respiratory processes have been linked to chronic respiratory diseases, most of which are preventable and treatable. Among these pathological conditions, rhinitis, bronchial asthma, chronic obstructive pulmonary disease, or COPD, and rhinomodeling due to exacerbated mucus production involving the epithelial mucosa of the upper and lower respiratory tract are commonly encountered. From these conditions, there is an exacerbated inflammatory capacity mediated by the cytokine cascade, with an emphasis on Tumor Necrosis Factor-α (TNF-α). This inflammatory cascade process predominantly presentes interferences such as persistent airway inflammation, tissue remodeling due to subepithelial fibrosis increasing the permeability of aggressive peptides, smooth muscle thickening, cell keratinization, and ultimately, respiratory tract cell hyperplasia, reducing the quality of life of affected individuals. In this worldwide pathological context, mites have been widely implicated in the development of allergic diseases. They are present in household dust environments, thus making mites globally recognized as allergens, as well as the protein from cells originating from the intestinal tract of this fauna's waste. Therefore, it is necessary to identify peptide sequences with allergenic capacity in the Tyr p2 protein of Tyrophagus putrescentiae. The prediction of potentially allergenic peptides, as well as the evaluation of their interaction energy with IgE, was performed through computational tools – molecular docking. These interactions allowed the synthesis of peptides predicted to have higher allergenic capacity, to be subsequently subjected to cellular assays to observe the inflammatory cascade generated by the predicted peptides
Descrição
Palavras-chave
Tyrophagus putrescentiae; Tyr p2; Der p2; Docking molecular; Rinite; Molecular Docking; Rhinitis
Citação
JUNG, Tatiana. Avaliação do potencial alergênico da proteína Tyr p2 do ácaro de de importância médica Tyrophagus putrescentiae. 2024. Dissertação (Mestrado) – Curso de Ciências Médicas, Universidade do Vale do Taquari - Univates, Lajeado, 29 abr. 2024. Disponível em: http://hdl.handle.net/10737/4847.