Estudo de um método de diagnóstico de Mycobacterium bovis baseado numa prática de biologia molecular com amostras de leite
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2018-08-08
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Lüdtke, Welton Everson
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Resumo
A tuberculose bovina é causada pelo Mycobacterium bovis e é considerada uma zoonose distribuída mundialmente sendo a espécie bovina o hospedeiro primário. Objetivos: Estudo do método de diagnóstico de Mycobacterium bovis em amostras de leite, confrontando seu resultado com o teste PPD (Purified Protein Derivative). Material e Métodos: Trata-se de um estudo experimental em 15 amostras de leite bovino in natura, oriundas de vacas leiteiras positivas ao teste da tuberculina de produtores de leite dos Vales do Taquari e Rio Pardo/RS. O método de diagnóstico de M. bovis utilizado foi a PCR em tempo real. A análise foi realizada no Laboratório de Biologia Molecular do Instituto de Pesquisas Veterinárias Desidério Finamor em Eldorado do Sul/RS. Resultados: Os resultados demonstraram a negatividade da presença de M.bovis em todas as amostras de leite analisadas. Conclusão: O presente estudo evidenciou a utilização da biologia molecular como alternativa de diagnóstico de tuberculose bovina, principalmente em amostras de leite. Uma doença que traz prejuízos aos proprietários e descrédito no mercado. Porém, o estudo não demostrou os achados
dos estudos validados na pesquisa do M. bovis no leite.
Bovine tuberculosis is caused by Mycobacterium bovis and is considered a zoonosis distributed worldwide and the bovine species is the primary host. Objectives: To study methods of diagnosis of Mycobacterium bovis in milk samples, comparing their results with the PPD (Purified Protein Derivative) test. Material and Methods: This is an experimental study in 15 samples of fresh bovine milk from dairy cows positive to the tuberculin test of milk producers of the Taquari and Rio Pardo / RS Valleys. The method of diagnosis of M. bovis used was the molecular biology test, real- time PCR. The analysis was carried out at the Laboratory of Molecular Biology of the Desidério Finamor Veterinary Research Institute in Eldorado do Sul / RS. Results: The results demonstrated the negativity of the presence of M.bovis in all analyzed milk samples. Conclusion: The present study evidenced the use of molecular biology as an alternative for the diagnosis of bovine tuberculosis, especially in milk samples. A disease that brings losses to the owners and discredit in the market. However, the study did not demonstrate the findings of studies validated in M. bovis research in milk.
Bovine tuberculosis is caused by Mycobacterium bovis and is considered a zoonosis distributed worldwide and the bovine species is the primary host. Objectives: To study methods of diagnosis of Mycobacterium bovis in milk samples, comparing their results with the PPD (Purified Protein Derivative) test. Material and Methods: This is an experimental study in 15 samples of fresh bovine milk from dairy cows positive to the tuberculin test of milk producers of the Taquari and Rio Pardo / RS Valleys. The method of diagnosis of M. bovis used was the molecular biology test, real- time PCR. The analysis was carried out at the Laboratory of Molecular Biology of the Desidério Finamor Veterinary Research Institute in Eldorado do Sul / RS. Results: The results demonstrated the negativity of the presence of M.bovis in all analyzed milk samples. Conclusion: The present study evidenced the use of molecular biology as an alternative for the diagnosis of bovine tuberculosis, especially in milk samples. A disease that brings losses to the owners and discredit in the market. However, the study did not demonstrate the findings of studies validated in M. bovis research in milk.
Descrição
Palavras-chave
Leite; Mycobacterium bovis; PCR; Tuberculose
Citação
STÜLP, Olivia. Estudo de um método de diagnóstico de Mycobacterium bovis baseado numa prática de biologia molecular com amostras de leite. 2017. Artigo (Graduação) – Curso de Biomedicina, Universidade do Vale do Taquari - Univates, Lajeado, 06 dez. 2017. Disponível em: http://hdl.handle.net/10737/2196.