Investigação da associação do polimorfismo do gene NFKB1 no risco de doença arterial coronariana
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Data
2017-10
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Orientador
Fallavena, Paulo Roberto Vargas
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Resumo
A principal doença cardiovascular é a doença arterial coronariana (DAC), cuja patologia é de origem inflamatória crônica, multifatorial, desencadeada por fatores genéticos, ambientais e de grande incidência mundial, apresentando, múltiplos estágios no desenvolvimento da aterosclerose. Os fatores de risco que predispõem o surgimento e o desencadeamento de DAC, são classificados como modificáveis e não-modificáveis. Por ser uma doença multifatorial, além da presença de fatores de risco para a patologia, diversos estudos têm demonstrado que o desenvolvimento da DAC tem forte influência de fatores genéticos, tais como os polimorfismos no gene NFKB, regulador mestre de ativação da cascata inflamatória. Por conseguinte, este estudo tem como objetivo a verificação da relação do polimorfismo NFKB -94 ATTG ins/del com a presença de DAC em indivíduos adultos, submetidos ao serviço de Hemodinâmica do Hospital Bruno Born de Lajeado, Rio Grande do Sul, Brasil. A amostra totalizou 324 indivíduos adultos de ambos os sexos. As amostras de sangue periférico foram coletadas para as dosagens bioquímicas e moleculares. Foi utilizada a técnica da reação em cadeia da polimerase (PCR) para a amplificação do polimorfismo, com primers em condições específicas e, posteriormente, foi realizada digestão enzimática para genotipagem do polimorfismo deste estudo, onde os fragmentos resultantes foram visualizados em gel de agarose 2%. Os testes estatísticos foram realizados através do software SPSS, versão 21.0. Para as frequências genotípicas, foi utilizado o teste do qui-quadrado de Pearson e as variáveis categóricas pelo método de Mann Whitney Kruskal-Wallis. Para o equilíbrio de Hardy-Weinberg foi utilizado o teste do qui-quadrado. Não foram identificadas associações significativas com nenhuma das variáveis investigadas. Os achados sugerem que o polimorfismo investigado não apresenta um papel significativo na doença arterial coronariana.
The main coronary artery diseases (CAD), whose pathology has a chronic inflammatory origin, multifactorial unleashed by genetic, environmental factors with a high incidence in the world. The risk factors that predispose the beginning and triggering of CAD are classified as modifiable and non-modifiable. As it is a multifactorial disease is known that besides the existence of risk factor for the pathology, numerous studies have been showing that the development of CAD has a strong influence on the genetic factors such as the polymorphism NFKB gene, main regulator of inflammatory cascade. This way, this study has as objective the verification of the relationship between -94 ATTG ins/del polymorphism of NFKB and the presence of CAD in adults, submitted through the Hemodinamic service of Bruno Born Hospital of Lajeado, Brazil. The sample of peripheral blood were collected for biochemistry and molecular analysis. The polymerase Chain Reaction (PCR) was used for the amplification of polymorphism, with primers in specific conditions, and later was done an enzymatic digestion for polymorphism genotyping -94 ATTG ins/del of gene NFKB1, where the resulting fragments were visualized on 2% agarose gel. The static test were done using the software SPSS version 21.0. for the genotype frequencies the chi-squared of Pearson test and the categorical variable by the method of Mann Whitney Kuskal Wallis were used. There was not found any significant association with any of the variables investigated. The findings suggest that the investigated polymorphism doesn’t presnt any significant role in the Coronary Artery Disease.
The main coronary artery diseases (CAD), whose pathology has a chronic inflammatory origin, multifactorial unleashed by genetic, environmental factors with a high incidence in the world. The risk factors that predispose the beginning and triggering of CAD are classified as modifiable and non-modifiable. As it is a multifactorial disease is known that besides the existence of risk factor for the pathology, numerous studies have been showing that the development of CAD has a strong influence on the genetic factors such as the polymorphism NFKB gene, main regulator of inflammatory cascade. This way, this study has as objective the verification of the relationship between -94 ATTG ins/del polymorphism of NFKB and the presence of CAD in adults, submitted through the Hemodinamic service of Bruno Born Hospital of Lajeado, Brazil. The sample of peripheral blood were collected for biochemistry and molecular analysis. The polymerase Chain Reaction (PCR) was used for the amplification of polymorphism, with primers in specific conditions, and later was done an enzymatic digestion for polymorphism genotyping -94 ATTG ins/del of gene NFKB1, where the resulting fragments were visualized on 2% agarose gel. The static test were done using the software SPSS version 21.0. for the genotype frequencies the chi-squared of Pearson test and the categorical variable by the method of Mann Whitney Kuskal Wallis were used. There was not found any significant association with any of the variables investigated. The findings suggest that the investigated polymorphism doesn’t presnt any significant role in the Coronary Artery Disease.
Descrição
Palavras-chave
NFKB; Doença Arterial Coronariana (DAC); Processo inflamatório; Aterosclerose; Doença cardiovascular
Citação
SEIBEL, Emily Bruch. Investigação da associação do polimorfismo do gene NFKB1 no risco de doença arterial coronariana. 2016. Artigo (Graduação) – Curso de Biomedicina, Universidade do Vale do Taquari - Univates, Lajeado, 09 dez. 2016. Disponível em: http://hdl.handle.net/10737/1687.